Here's what ya do:
Table One
| Run 1 | Run 2 | Run 3 | Run 4 | Run 5 | |
| Fe(NO3)3 | 5.0 mL | 5.0 mL | 5.0 mL | 5.0 mL | 5.0 mL |
| KSCN | 1.0 mL | 2.0 mL | 3.0 mL | 4.0 mL | 5.0 mL |
| H2O | 4.0 mL | 3.0 mL | 2.0 mL | 1.0 mL | 0.0 mL |
1. Set up 5 test tubes and label them 1-5.
2. Pour 30 mL 2.00 x 10-3M Fe(NO3)3 in 1 M HNO3 into a 100 mL beaker. Pipet 5.0 mL of this solution into each test tube.
3. Add 20 mL 2.00 x 10-3 M KSCN to another 100 mL beaker. Pipet 1, 2, 3, 4, and 5 mL into the corresponding test tubes (as indicated in Table1).
4. Pipet the corresponding amount of water into each test tube, as per the table.
5. Mix each solution. The colors can be seen in the picture below:
6. Calibrate the spectrophotometer (Spec20): (1) With no cell, set the transmittance to zero. (2) Set wavelength to 447 nm. (3) Place a blank in the chamber and set the absorbance to zero. A Spec20 can be seen below:
7. Place some of each mixture into spectrophotometer tubes (about two-thirds full) and measure the absorbance at 447 nm. Use the calibration curve to determine the concentration of FeSCN2+ as explained in the Beer-Lambert section. A proper loading procedure can be seen by the following picture:
8. Complete the data sheet and return the spectrophotometer tubes.
9. All liquids should be desposed by however the instructor directs.